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标记抗体服务

 

但是这些成果了我       占其门店总数的80%

 

服务宗旨:高品质的标记原料采购,规范的标记操作流程,严格的标记产品质控,合理的市场价格定位。
服务目标:以国际高标准的标记服务水平,提供高品质的标记抗体产品,助力科研人员轻松科研。



一、服务内容:

本服务项目主要为接收客户的抗体、蛋白、多肽、小分子化合物进行常规标记物标记。

1. 可接收的待标记样品包含抗体IgG、大分子蛋白、小分子多肽、小分子化合物等。

2. 可提供标记服务的标记物有:

A. 酶标记物:碱性磷酸酶(AP/ALP),辣根过氧化物酶(HRP);

B. 生物素(Biotin);

C. 常规荧光素:FITC,Rhodamine B(RBITC);

D. Cy系列荧光素:Cy3,Cy5,Cy5.5, Cy7;

E. Alexa Fluor系列荧光素:AF350,AF488,AF555,AF594,AF647,AF680,AF750;

F. 荧光蛋白:藻红蛋白(PE),别藻蓝蛋白(APC),PerCP;

G. 荧光双标记:PE-Cy3,PE-Cy5,PE-Cy5.5,PE-Cy7;

H. 胶体金标记Co-Au;

二、服务流程:

1.  客户提交委托标记请求并明确标记要求,与公司商定具体的实验项目、数量和费用,双方签订《委托标记制备合同》。

2.  客户提供待标记样品,我公司按照合同要求安排标记服务,并在规定时间内完成标记委托。

3.  标记完成后,我公司按照客户要求分装,免费低温邮寄给客户,同时提供标记报告。

三、样品要求:

1.  待标记样品需保证90%以上的纯度,样品中不含有干扰标记物结合的成分,如有特殊成分,请提前标注说明。

2.  待标记样品最小标记量为1mg,最好是干粉,如果是液体,浓度应大于1mg/ml。

3.  抗体IgG样品中应不含BSA、叠氮钠、甘氨酸等成分。

4.  大分子蛋白应提供分子量、等电点、缓冲液成分及pH等信息;小分子多肽需提供氨基酸序列;小分子化合物还需提供分子结构式。

5.  请提前说明任何特殊情况。

四、收费标准:

1.  抗体和大分子蛋白标记收费标准:

 标记物  类型  标记物厂家  标记数量  周期
 1-2mg  2-5mg  5-10mg
 HRP    Sigma  1200  1600  2500  10天
 AP    Invitrogen  2000  2800  5000  10天
 Biotin  生物素  Sigma  1000  1500  2000  10天
 FITC  荧光  Sigma  1000  1500  2000  10天
 RBITC  荧光  Sigma  1200  1600  2500  10天
 Cy系列  荧光  Invitrogen  1500  2200  3200  10天
 Alexa Fluor  荧光  Life Technologies  2000  2500  4000  10天
 PE  荧光  GE  1600  2400  3200  10天
 APC  荧光  GE  1600  2400  3200  10天
 PerCP  荧光  GE  1600  2400  3200  10天
 PE-Cy双标  荧光  GE& Invitrogen  3000  4800  6400  15天
 Co-Au  胶体金  Sigma  1500  2500  4000  10天

 

五、标记物介绍:
碱性磷酸酶(alkaline phosphatase,ALP或AP)是免疫诊断试剂产品最常用的标记酶之一,优点是稳定性高、灵敏度高。ALP是一种能够将对应底物去磷酸化的酶,即通过水解磷酸单酯将底物分子上的磷酸基团除去,并生成磷酸根离子和自由的羟基,这类底物包括核酸、蛋白、生物碱等。而该脱去磷酸基团的过程被称为去磷酸化或脱磷酸化。碱性磷酸酶是磷酸酶的一种,磷酸酶的作用与激酶的作用正相反,激酶是磷酸化酶,可以利用能量分子,如ATP,将磷酸基团加到对应底物分子上。

辣根过氧化物酶(Horseradish Peroxidase,HRP)活性高,稳定,分子量小,纯酶容易制备,所以最常用。HRP广泛分布于植物界,辣根中含量高,它是由无色的酶蛋白和棕色的铁卟啉结合而成的糖蛋白,糖含量18%。HRP由多个同功酶组成,分子量为40,000,等电点为PH3~9,酶催化的最适PH因供氢体不同而稍有差异,但多在PH5左右。酶溶于水和58%以下饱和度硫酸铵溶液。HRP的辅基和酶蛋白最大吸收光谱分别为403nm和275nm,一般以OD403nm /OD275nm的比值RZ(德文ReinheitZahl)表示酶的纯度。高纯度的酶RZ值应在3.0左右(最高可达3.4)。RZ值越小,非酶蛋白就越多。

FITC和RhodamineB(RBITC)是常见的荧光素,性质较稳定;

Cy系列荧光染料由于荧光较强,对光猝灭有抵抗力而荧光不宜消退,近年来应用比较广泛;

六、常用荧光素信息:


北京兴发娱乐首页官网 标记抗体发表文献:

文献名称:The necrosome promotes pancreatic oncogenesis via CXCL1 and Mincle-induced immune suppression.

期刊名称:Nature. 2016 Apr 14;532(7598):245-9. doi: 10.1038/nature17403. Epub 2016 Apr 6.

影响因子:(IF)41.456

产品应用:bs-5805R-FITC | RIPK-1 Polyclonal Antibody, FITC Conjugated | IHC-F

产品应用:bs-7596R-A647 | RIP3 Polyclonal Antibody, Alexa Fluor® 647 Conjugated | IHC-F

研究机构:S. Arthur Localio Laboratory, Department of Surgery, New York University School of Medicine; Department of Cell Biology, New York University School of Medicine; Department of Pathology, New York University School of Medicine; Cold Spring Harbor Laboratories.

文献链接:http://www.ncbi.nlm.nih.gov/pubmed/27049944


文献名称:Protein kinase A determines timing of early differentiation through epigenetic regulation with G9a.

期刊名称:Cell Stem Cell. 2012 Jun 14;10(6):759-70. doi: 10.1016/j.stem.2012.02.022.

影响因子:(IF)25.315

产品应用:bs-0008R-PE | Rabbit Anti-Nestin Polyclonal Antibody, PE Conjugated | FCM

产品应用:bs-2358R-PE | Rabbit Anti-FOXA2/HNF3 beta Polyclonal Antibody, PE Conjugated | FCM

研究机构:Department of Stem Cell Differentiation, Institute for Frontier Medical Sciences, Kyoto University; Laboratory of Reproductive Biology, Graduate School of Agriculture; Experimental Research Center for Infectious Disease, Institute for Virus Research,Kyoto University; Cancer Institute, Japanese Foundation for Cancer Research, Tokyo.

文献链接:http://www.ncbi.nlm.nih.gov/pubmed/22704517


文献名称:Transcriptional profiling of mouse B cell terminal differentiation defines a signature for antibody-secreting plasma cells.

期刊名称:Nat Immunol. 2015 Jun;16(6):663-73. doi: 10.1038/ni.3154. Epub 2015 Apr 20.

影响因子:(IF)24.973

产品应用:bs-9425R-PE | Rabbit Anti-TSPAN15 Polyclonal Antibody, PE Conjugated | FCM

研究机构:The Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria, Australia; Department of Computing and Information Systems, The University of Melbourne; Department of Medical Biology, The University of Melbourne; Department of Pathology, The University of Melbourne; Department of Microbiology & Immunology, The University of Melbourne;

文献链接:http://www.ncbi.nlm.nih.gov/pubmed/25894659


文献名称:Vagal Regulation of Group 3 Innate Lymphoid Cells and the Immunoresolvent PCTR1 Controls Infection Resolution.

期刊名称:Immunity. 2017 Jan 17;46(1):92-105. doi: 10.1016/j.immuni.2016.12.009. Epub 2017 Jan 5.

影响因子:(IF)24.08

产品应用:bs-6505R-A647 | Rabbit Anti-15 Lipoxygenase 1 antibody,Alexa Fluor®647 Conjugated | FCM

研究机构:Center for Experimental Therapeutics and Reperfusion Injury, Harvard Institutes of Medicine, Department of Anesthesiology, Perioperative and Pain Medicine, Brigham and Women's Hospital and Harvard Medical School, Boston, MA 02115, USA; Lipid Mediator Unit, Centre for Biochemical Pharmacology, William Harvey Research Institute, Barts and the London School of Medicine, Queen Mary University of London, London EC1M 6BQ, UK.

文献链接:http://www.ncbi.nlm.nih.gov/pubmed/28065837


文献名称:Pten loss in the bone marrow leads to G-CSF-mediated HSC mobilization.

期刊名称:J Exp Med. 2013 Oct 21;210(11):2337-49. doi: 10.1084/jem.20122768. Epub 2013 Oct 14.

影响引子:(IF)13.912

产品应用:bs-2478R-PE | Rabbit Anti-CCL12/MCP5 Polyclonal antibody, PE Conjugated | FCM

研究机构:Deutsches Krebsforschungszentrum (DKFZ); Heidelberg Institute for Stem Cell Technology and Experimental Medicine; Swiss Institute for Experimental Cancer Research (ISREC), School of Life Science, Ecole Polytechnique Fédérale de Lausanne (EPFL); Department of Molecular and Medical Pharmacology, Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research; Department of Hematology, Universitätsklinikum Essen, Westdeutsches Tumorzentrum (WTZ); Department of Laboratory Medicine and Comprehensive Cancer Center, University of California

文献链接:http://www.ncbi.nlm.nih.gov/pubmed/24127490


文献名称:Identification of blood vascular endothelial stem cells by the expression of protein C receptor.

期刊名称:Cell Res. 2016 Oct;26(10):1079-1098. doi: 10.1038/cr.2016.85. Epub 2016 Jul 1.

影响引子:(IF)12.41

产品应用:bs-4800R-A647 | Rabbit Anti-NG2 Polyclonal antibody,Alexa Fluor® 647 Conjugated | FCM

研究机构:The State Key Laboratory of Cell Biology, CAS Center for Excellence in Molecular Cell Science, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China.

文献链接:http://www.ncbi.nlm.nih.gov/pubmed/27364685


文献名称:Endothelial to mesenchymal transition is common in atherosclerotic lesions and is associated with plaque instability.

期刊名称:Nat Commun. 2016 Jun 24;7:11853. doi: 10.1038/ncomms11853.

影响引子:(IF)11.33

产品应用:bs-5760R-A647 |Rabbit Anti-FAP1Polyclonalantibody,ALexaFluor® 647 Conjugated | FCM

产品应用:bs-5758R-A647 |Rabbit Anti-FAPAPolyclonalantibody,ALexaFluor ® 647 Conjugated | FCM 

研究机构:The Zena and Michael A. Wiener Cardiovascular Institute, Icahn School of Medicine at Mount Sinai, New York, New York 10029, USA. Department of Genetics and Genomic Sciences and Icahn Institute for Genomics and Multiscale Biology, Icahn School of Medicine at Mount Sinai, New York, New York 10029, USA.Trialomics LLC, Seattle, Washington 98115, USA. Department of Pathology and Immunology, Washington University, St Louis, Missouri 63110, USA. Brigham and Women's Health Care, Boston, Massachusetts 02115, USA. Marie-Josée and Henry R. Kravis Cardiovascular Health Center, Icahn School of Medicine at Mount Sinai, New York, New York 10029, USA. Centro Nacional de Investigaciones Cardiovasculares (CNIC), 28029 Madrid, Spain. Center for Molecular Medicine, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland, 20892, USA.

文献链接:http://www.ncbi.nlm.nih.gov/pubmed/27340017



文献名称:Macrophage-derived extracellular vesicle-packaged WNTs rescue intestinal stem cells and enhance survival after radiation injury.

期刊名称:Nat Commun. 2016 Oct 13;7:13096. doi: 10.1038/ncomms13096.

影响引子:(IF)11.33

产品应用:bs-1948R-Biotin | Rabbit Anti-WNT5A polyclonal antibody, BiotinConjugated | ELISA

产品应用:bs-1933R-Biotin |Rabbit Anti-WNT9Apolyclonalantibody, BiotinConjugated| ELISA

研究机构:Department of Radiation Oncology, Albert Einstein College of Medicine &Montefiore Medical Center, Bronx, New York 10461, USA. Department of Developmental and Molecular Biology, Albert Einstein College of Medicine, Bronx, New York 10461, USA. Department of Medicine, Division of Digestive and Liver Diseases, Irving Cancer Research Center, Columbia University, New York, New York 10032, USA. Department of Pathology and Laboratory Medicine, University of Kansas Medical Center, Kansas City, Kansas 66160, USA. Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, New York, 10461, USA. Department of Pathology, Albert Einstein College of Medicine, Bronx, New York 10461, USA. MRC Centre for Reproductive Health, University of Edinburgh, Edinburgh EH16 4TJ, UK

文献链接:http://www.ncbi.nlm.nih.gov/pubmed/27734833



文献名称:A TNFRSF14-FcɛRI-mast cell pathway contributes to development of multiple features of asthma pathology in mice.

期刊名称:Nat Commun. 2016 Dec 16;7:13696. doi: 10.1038/ncomms13696.

影响引子:(IF)11.33

产品应用:bs-2462R-PE |Rabbit Anti-TNFSF14 polyclonal antibody, PEConjugated | FCM

研究机构:Department of Pathology &Department of Genetics &Department of Pediatrics &Department of Microbiology and Immunology and Sean N. Parker Center for Allergy and Asthma Research, Stanford University School of Medicine, Stanford, California 94305, USA. Department of Immunology, Unit of Antibodies in Therapy and Pathology, INSERM U1222, Institut Pasteur, Paris 75015, France. CeMM Research Center for Molecular Medicine of the Austrian Academy of Sciences and Department of Medicine I, Research Laboratory of Infection Biology, Medical University of Vienna, Vienna 1090, Austria. Division of Developmental Immunology, La Jolla Institute for Allergy and Immunology, La Jolla, California 92037, USA. Institute for Immunology, Technische Universität Dresden, Dresden 01307, Germany.

文献链接:http://www.ncbi.nlm.nih.gov/pubmed/27982078


文献名称:HCV induced reduction in miR‐181a impairs CD4+ T cell responses via over‐expression of DUSP6.

期刊名称:Hepatology. 2015 Apr;61(4):1163-73. doi: 10.1002/hep.27634. Epub 2015 Feb 4.

影响引子:(IF)11.19

产品应用:bs-11546R-FITC |Rabbit Anti-DUSP6Polyclonalantibody, FITCConjugated | FCM

研究机构:Center for Inflammation, Infectious Disease and Immunity, James H. Quillen College of Medicine, East Tennessee State University, Johnson City, TN.

文献链接:http://www.ncbi.nlm.nih.gov/pubmed/25477247



文献名称:Neutralization of pro‐inflammatory monocytes by targeting TLR2 dimerization ameliorates colitis.

期刊名称:EMBO J. 2016 Mar 15;35(6):685-98. doi: 10.15252/embj.201592649. Epub 2016 Feb 15.

影响引子:(IF)10.43

产品应用:bs-2716R-PE |Rabbit Anti-TLR6Ployclonalantibody,PEConjugated| FCM

研究机构:Department of Biological Chemistry, The Weizmann Institute of Science, Rehovot, Israel. Department of Immunology, The Weizmann Institute of Science, Rehovot, Israel. Department of Biological Services, The Weizmann Institute of Science, Rehovot, Israel.  Department of Veterinary Resources, The Weizmann Institute of Science, Rehovot, Israel. 

文献链接:http://www.ncbi.nlm.nih.gov/pubmed/26884587  


文献名称:Multistage NanovehicleDelivery System Based on Stepwise Size Reduction and Charge Reversal for Programmed Nuclear Targeting of Systemically Administered Anticancer Drugs.

期刊名称:Advanced Functional Materials.25(26).DOI: 10.1002/adfm.201501248.July 8, 2015

影响引子:(IF)10.40

产品应用:bs-0293G-FITC |Goat Anti-rat IgG antibody, FITCConjugated | Other

研究机构:Key Laboratory of Drug Targeting and Drug Delivery System, Ministry of Education, West China School of Pharmacy, Sichuan University, Chengdu, P. R. China 

文献链接:http://onlinelibrary.wiley.com/doi/10.1002/adfm.201501248/pdf



文献名称:Therapeutic effects of glatiramer acetate and grafted CD115+ monocytes in a mouse model of Alzheimers disease.

期刊名称:Brain. 2015 Aug;138(Pt 8):2399-422. doi: 10.1093/brain/awv150.Epub 2015 Jun 6.

影响引子:(IF)10.23

产品应用:bs-0397R-A488 |Rabbit Anti-MMP9Plyclonalantibody,Alexa Fluor®488 Conjugated | FCM

研究机构:Department of Neurosurgery, Maxine-Dunitz Neurosurgical Institute, Cedars-Sinai Medical Centre, Los Angeles, CA 90048, USA.Widjaja Foundation Inflammatory Bowel and Immunobiology Research Institute, Cedars-Sinai Medical Centre, Los Angeles, CA 90048, USA. Department of Biomedical Sciences, Cedars-Sinai Medical Centre, Los Angeles, CA 90048, USA

文献链接:http://www.ncbi.nlm.nih.gov/pubmed/26049087



 

法律声明:The Alexa Fluor dye antibody conjugates in this product are sold under license from Life Technologies Corporation for research use only, except for use in combination with DNA microarrays. The Alexa Fluor® dyes (except for Alexa Fluor® 430 dye) are covered by pending and issued patents. Alexa Fluor® is a registered trademark of Molecular Probes, Inc. U.S. Patent No. 5,675,063.LINK

 

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