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Rabbit Anti-beta-Actin (Loading Control) antibody (bs-0061R)
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产品编号 bs-0061R
英文名称 beta-Actin (Loading Control)
中文名称 β-肌动蛋白/β-Actin 抗体(内参抗体)
别    名 Beta Actin; beta-Actin; ACTB; Actin cytoplasmic 1; Actin, beta; Beta actin; beta cytoskeletal actin;A X actin like protein; ACTB; Actin cytoplasmic 1; alpha sarcomeric Actin; Actx; Beta cytoskeletal actin; Melanoma X actin; PS1TP5BP1; ACTB_HUMAN.  
Journal
PMID
IF
Application
Biomaterials (2013)
7.6040
WB
Biomaterials (2013)
7.6040
WB
Nanotoxicology (2015)
6.4110
WB
Arteriosclerosis, thrombosis, and vascular biology (2008)
6.3380
WB
Archives of toxicology (2012)
5.2150
WB
Molecular Neurobiology (2015)
5.1370
WB
Molecular Cancer (2013)
5.1300
WB
British Journal of Pharmacology (2014)
5.0670
WB
Endocrinology (2015)
4.5030
WB
Molecular Pharmacology (2013)
4.4110
WB
Cancer letters (2007)
4.2580
WB
Biochimica et Biophysica Acta (BBA)-Molecular and Cell Biology of Lipids (2010)
4.1340
WB
Nanotechnology (2013)
3.8420
WB
Nanotechnology (2013)
3.8420
WB
Proteomics (2016)
3.8100
WB
The Journal of Nutritional Biochemistry (2014)
3.7940
WB
PloS One (2012)
3.7300
WB
PloS One (2013)
3.7300
WB
PloS One (2013)
3.7300
WB
Cancer Immunology, Immunotherapy (2013)
3.6370
WB
Colloids and Surfaces B: Biointerfaces (2012)
3.5540
WB
PLoS One (2015)
3.5340
WB
Cellular Physiology and Biochemistry (2014)
3.4150
WB
Free radical research (2007)
3.2790
WB
Developmental & Comparative Immunology (2012)
3.2380
WB/IP
Developmental & Comparative Immunology (2013)
3.2380
WB
Developmental & Comparative Immunology (2013)
3.2380
WB
Developmental & Comparative Immunology (2013)
3.2380
WB
Developmental & Comparative Immunology (2012)
3.2380
WB
International Journal of Biological Sciences (2014)
3.1680
WB
Toxicology Letters (2011)
3.1450
WB
Neuroscience (2011)
3.1220
WB
Diabetes/Metabolism Research and Reviews (2012)
2.9680
WB
Chemico-Biological Interactions (2012)
2.9670
WB
Fish & Shellfish Immunology (2012)
2.9640
WB
Journal of Cancer Research and Clinical Oncology (2012)
2.9140
WB
Food and Chemical Toxicology (2016)
2.9000
WB
Brain Research Bulletin (2016)
2.7180
WB
Life Sciences (2016)
2.7020
WB
Nutrition and Cancer (2014)
2.6950
WB
Neurochemistry international (2013)
2.6590
WB
Neurochemistry international (2013)
2.6590
WB
Neurochemistry international (2011)
2.6590
WB
Life Science Journal (2013)
2.5550
WB
Toxicology and Applied Pharmacology (2016)
2.5320
WB
Tumor Biology (2013)
2.5180
WB
Acta Pharmacologica Sinica (2015)
2.4960
WB
World Journal of Gastroenterology (2015)
2.4330
WB
Biochemical and Biophysical Research Communications (2013)
2.4060
WB
Acta Pharmacologica Sinica (2014)
2.3540
WB
Acta Pharmacologica Sinica (2014)
2.3540
WB
Molecular and Cellular Biochemistry (2011)
2.3290
WB
Molecular and Cellular Biochemistry (2014)
2.3290
WB
Rheumatology international (2010)
2.2140
WB
Medical Oncology (2013)
2.1470
WB
Reproductive Biology and Endocrinology (2013)
2.1400
WB
Neurochemical Research (2013)
2.1250
WB
Virology Journal (2011)
2.0900
WB
Respiratory physiology & neurobiology (2013)
2.0510
WB
International Journal of Clinical and Experimental Pathology (2015)
1.8910
WB
Research in Veterinary Science (2012)
1.7740
WB
Cellular Immunology (2013)
1.7430
WB
Applied Biochemistry and Biotechnology (2016)
1.7400
WB
Poultry Science (2014)
1.7280
WB
Nephrology (2011)
1.6880
WB
Biological Trace Element Research (2015)
1.6080
WB
Biological trace element research (2013)
1.3070
WB
1.2770
WB
Brazilian Journal of Medical and Biological Research (2014)
1.1390
WB
Experimental & Clinical Cardiology (2013)
1.1000
WB
World Journal of Surgical Oncology (2014)
1.0900
WB
CHINESE MEDICAL JOURNAL-BEIJING-ENGLISH EDITION (2009)
0.9010
WB
Pediatrics International (2016)
0.7300
WB
Experimental and Therapeutic Medicine (2014)
0.3440
WB
规格价格 50ul/580元 这是一个被广泛供认的现实    100ul/920元 容易被防空导弹和航空导弹攻击    200ul/1600元 通过在新一代308车型上的应用    大包装/询价
说 明 书 这对全球动力革新发生深远影响  在一带一路战略方案中  ????1个月前
产品类型 从2005年开端 
研究领域 我们在这边的时候就认识  李敏成婚今后才搬出了中南海  当还不能够用语言表达的时分  作为群众最大的赢利贡献者  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Rabbit, Sheep, Bee, Fish, Guinea Pig, Hamster, Goat, Cat, mt,op
产品应用 WB=1:5000-20000 ELISA=1:5000-20000 IHC-P=1:500-1000 IHC-F=1:500-1000 Flow-Cyt=1μg/Test IF=1:100-1000 可能对设备造成了必定损坏
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 42kDa
细胞定位 细胞浆 
性    状 Lyophilized or Liquid
浓    度 1mg/1ml
免 疫 原 Synthetic MAP peptide derived from human beta-Actin
亚    型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
生 产 商 BIOSS
PubMed PubMed
产品介绍 background:
Loading Control
This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. [provided by RefSeq, Jul 2008].

Function:
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

Subunit:
Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others. Identified in a mRNP granule complex, at least composed of ACTB, ACTN4, DHX9, ERG, HNRNPA1, HNRNPA2B1, HNRNPAB, HNRNPD, HNRNPL, HNRNPR, HNRNPU, HSPA1, HSPA8, IGF2BP1, ILF2, ILF3, NCBP1, NCL, PABPC1, PABPC4, PABPN1, RPLP0, RPS3, RPS3A, RPS4X, RPS8, RPS9, SYNCRIP, TROVE2, YBX1 and untranslated mRNAs. Component of the BAF complex, which includes at least actin (ACTB), ARID1A, ARID1B/BAF250, SMARCA2, SMARCA4/BRG1, ACTL6A/BAF53, ACTL6B/BAF53B, SMARCE1/BAF57 SMARCC1/BAF155, SMARCC2/BAF170, SMARCB1/SNF5/INI1, and one or more of SMARCD1/BAF60A, SMARCD2/BAF60B, or SMARCD3/BAF60C. In muscle cells, the BAF complex also contains DPF3. Found in a complex with XPO6, Ran, ACTB and PFN1. Component of the MLL5-L complex, at least composed of MLL5, STK38, PPP1CA, PPP1CB, PPP1CC, HCFC1, ACTB and OGT. Interacts with XPO6 and EMD. Interacts with ERBB2.

Subcellular Location:
Cytoplasm. cytoskeleton.

Tissue Specificity:
Ubiquitously expressed in all eukaryotic cells.

Post-translational modifications:
ISGylated.
Oxidation of Met-44 by MICALs (MICAL1, MICAL2 or MICAL3) to form methionine sulfoxide promotes actin filament depolymerization. Methionine sulfoxide is produced stereospecifically, but it is not known whether the (S)-S-oxide or the (R)-S-oxide is produced.

DISEASE:
Defects in ACTA1 are the cause of nemaline myopathy type 3 (NEM3) [MIM:161800]. A form of nemaline myopathy. Nemaline myopathies are muscular disorders characterized by muscle weakness of varying severity and onset, and abnormal thread-or rod-like structures in muscle fibers on histologic examination. The phenotype at histological level is variable. Some patients present areas devoid of oxidative activity containg (cores) within myofibers. Core lesions are unstructured and poorly circumscribed.
Defects in ACTA1 are a cause of myopathy congenital with excess of thin myofilaments (MPCETM) [MIM:161800]. A congenital muscular disorder characterized at histological level by areas of sarcoplasm devoid of normal myofibrils and mitochondria, and replaced with dense masses of thin filaments. Central cores, rods, ragged red fibers, and necrosis are absent.

Similarity:
Belongs to the actin family.

SWISS:
P60709

Gene ID:
60

Database links:

Entrez Gene: 396526 Chicken

Entrez Gene: 60 Human

Entrez Gene: 11461 Mouse

Entrez Gene: 100009272 Rabbit

Entrez Gene: 81822 Rat

Omim: 102630 Human

SwissProt: P60706 Chicken

SwissProt: P60712 Cow

SwissProt: P60708 Horse

SwissProt: P60709 Human

SwissProt: P60710 Mouse

SwissProt: P29751 Rabbit

SwissProt: P60711 Rat

SwissProt: P60713 Sheep

Unigene: 520640 Human

Unigene: 708120 Human

Unigene: 727576 Human

Unigene: 328431 Mouse

Unigene: 391967 Mouse

Unigene: 94978 Rat



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

内参抗体
β-Actin是横纹肌肌纤维中的一种主要蛋白质成分,也是肌肉细丝及细胞骨架微丝的主要成分。具有收缩功能,分布广泛,具有高度保守性,在细胞中的表达相对稳定,因此常被用作校正系统的内参。β-Actin分子量为42 kDa,
此抗体主要用于标记平滑肌及其来源的肿瘤。
我公司开发的β-Actin抗体已被国内外广大科研工作者使用,被称谓:质量信得过产品.
产品图片
Sample:
A549 Cell (Human) Lysate at 30 ug
Primary: Lane1: Anti-beta-Actin (bs-0061R) at 1/500 dilution
Lane2: Anti-beta-Actin (bs-0061R) at 1/1000 dilution
Lane3: Anti-beta-Actin (bs-0061R) at 1/2000 dilution
Lane4: Anti-beta-Actin (bs-0061R) at 1/4000 dilution
Lane5: Anti-beta-Actin (bs-0061R) at 1/5000 dilution
Lane6: Anti-beta-Actin (bs-0061R) at 1/8000 dilution
Lane7: Anti-beta-Actin (bs-0061R) at 1/10000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 42 kD
Observed band size: 47 kD
Sample:
A549 Cell (Human) Lysate at 30 ug
Primary: Lane1: Anti-beta-Actin (bs-0061R) at 1/500 dilution
Lane2: Anti-beta-Actin (bs-0061R) at 1/1000 dilution
Lane3: Anti-beta-Actin (bs-0061R) at 1/2000 dilution
Lane4: Anti-beta-Actin (bs-0061R) at 1/4000 dilution
Lane5: Anti-beta-Actin (bs-0061R) at 1/5000 dilution
Lane6: Anti-beta-Actin (bs-0061R) at 1/8000 dilution
Lane7: Anti-beta-Actin (bs-0061R) at 1/10000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 42 kD
Observed band size: 47 kD
Sample:
Lane1: 293T Cell Lysate at 25 ug
Lane2: A549 Cell Lysate at 25 ug
Lane3: A431 Cell Lysate at 25 ug
Primary: Anti- beta-Actin (bs-0061R) at 1/1000 and 1/5000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 42kD
Observed band size: 42 kD
Sample: Lung lysate at 30ug;
Primary: Anti-beta-actin (bs-0061R) at 1:1000 dilution
Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bse-0295G) at 1:3000 dilution
Predicted band size : 42kD
Observed band size : 42kD
Tissue/cell: human cervical carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Beta-actin Polyclonal Antibody, Unconjugated(bs-0061R) 1:1500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Blank control: RSC96(blue).
Primary Antibody: Rabbit Anti-beta-Actin /FITC Conjugated antibody (bs-0061R/FITC), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG/FITC(orange) ,used under the same conditions.
Protocol
The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice. The cells were washed twice with 1 X PBS. The cells were incubated in 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions followed by the incubated with antibody (bs-0061R/FITC, 1μg /1x10^6 cells) for 30 min on ice. Acquisition of 20,000 events was performed.
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